Ecoli Strain for protein expression

SignalT5 signalt5 at aol.com
Tue Aug 8 21:44:30 EST 1995


I had a similar problem getting expression of a full length GST fusion. 
After several failures with protease deficient strains of E. coli, the
problem completely disappeared with the TOPP3 strain sold as competent
cells by Stratagene.  This strain produced good yields of full length
fusion protein and only small amounts of  lower molecular weight material.
The other TOPP strains worked less well for the protein I was trying to
express.

Jack Lichy
Armed Forces Institute of Pathology
Washington, DC



More information about the Methods mailing list