help! pcr of mtDNA control region
Julia54601
julia54601 at aol.com
Tue Aug 8 20:32:06 EST 1995
Our lab has a strange result from the pcr of a 500 bp region that contains
short repeat sequences. Although we a pcr-ing from an isolated fragment,
we are getting a heavy smear above the anticipated product. Very large --
right up to the wells. We tried making the pcr more stringent and it
won't go away. Has anyone ever heard of repeat sequences acting as
self-primers, sort of stringing products together? Any advice or
speculations? Please send e-mail to my address: Julia54601 at aol.com.
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