RNA gel help
STEVE MADISON
g5300013 at nickel.laurentian.ca
Tue Aug 8 14:25:46 EST 1995
hello,
i have been getting some pretty wierd migration patterns while running a
formaldehyde gel electrophoresis of RNA. it looks like a wine glass in that
it narrows between the 5s and 18s bands. has anyone experienced this before?
i was just wondering how important the current is to running a successful RNA
electrophoresis gel. according to Protocols in Molecular Biology the voltage
should be 5 V/cm but no mention is made of the proper current setting. are
there any rules of thumb? is there a proper current? any help is greatly
appreciated.
steve madison
neuromuscular research
laurentian university
g5300013 at nickel.laurentian.ca
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