Annealing oligos for adapter preparation
brad at corona.med.utah.edu
Wed Aug 9 16:35:57 EST 1995
In article <408d6j$p00 at news.tamu.edu>, dilip <dilip at zeus.tamu.edu> wrote:
> Dear netters,
>I need to make an adapter and I have oilgos for the same.
Check out Stover, Vodkin & Oaks. (1987). Use of Conversion Adaptors to
Clone Antigen Genes in lambda gt11. Analytical Biochemistry. 163:398-407.
It's really simple, add your two oligo's in equal portions, adjust the
concentration to 20 uM in 25 mM Tris-HCl, 8.0, 10 mM MgCl2. Heat to 95 C,
and cool to room temperature over 1 hour. (Quoted without permission from
Stover, et. al.)
Brad Nicholson |"If it worked the first time, it wouldn't be
Department of Pathology | research."...Brad Nicholson
University of Utah | Live from behind the Zion Curtian.
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or: (801)-581-4365 | My opinions are solely my own.
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