RNase protection probe activities

Colin MacD macdiarm at sbsu1.auckland.ac.nz
Wed Aug 9 14:45:31 EST 1995

I am setting up an RNAse protection assay and have been having problems with degradation (i.e. radiolytic decay) of my 32P-labelled probes. My probes have specific activities of about 5x10^9 cpm/microgram. I dont get degradation using low sp. activity probes, but need high sensitivity.
Is 5x10^9 too hot?
What is the best probe size and activity for high sensitivity?
I would greatly appreciate any help.

Dave Whittaker
whittakr at sbsu1.auckland.ac.nz

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