Help recovering 200bp DNA fragment

Bernard Murray murrayb at dc37a.nci.nih.gov
Wed Aug 9 13:53:58 EST 1995


In article <Pine.SUN.3.91.950808131722.24674A-100000 at chuma>, 
leverone at CHUMA.CAS.USF.EDU says...

[Request for help deleted]
>
>Harshinee:  Try something to increase ppt. of the DNA such as glycogen, a 
>20 ug/ml soln. helps.  Add 1 uL per 50 uL of DNA soln.  Marianne, USF, 
>Tampa, FL
>

Just wondering,
	Sigma offers 7 different types of glycogen (two of them being
pretty expensive).  Does it matter which type you use for this purpose?
Is there a nuclease-free version or can you cook it up to kill them off
(as for DNase free RNase)?
			Bernard
bernard at elsie.nci.nih.gov




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