Help recovering 200bp DNA fragment
Bernard Murray
murrayb at dc37a.nci.nih.gov
Wed Aug 9 13:53:58 EST 1995
In article <Pine.SUN.3.91.950808131722.24674A-100000 at chuma>,
leverone at CHUMA.CAS.USF.EDU says...
[Request for help deleted]
>
>Harshinee: Try something to increase ppt. of the DNA such as glycogen, a
>20 ug/ml soln. helps. Add 1 uL per 50 uL of DNA soln. Marianne, USF,
>Tampa, FL
>
Just wondering,
Sigma offers 7 different types of glycogen (two of them being
pretty expensive). Does it matter which type you use for this purpose?
Is there a nuclease-free version or can you cook it up to kill them off
(as for DNase free RNase)?
Bernard
bernard at elsie.nci.nih.gov
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