Ni-NTA and EDTA?

To: Nikolai troianovsk_s at MSDISK.WUSTL.EDU
Wed Aug 9 12:45:32 EST 1995


>
From: Frank.Neumann at uni-konstanz.de (Frank Neumann)
>

>
Hi,
>
has anyone experience, up to what concentrations EDTA does not effect the
>
binding of 6xHis Proteins to Ni-NTA?
>
In detail: what about 1mM EDTA?
>

>
Stephan
>


Hi, Stephan.

I think that up to 1mM of EDTA would not affect binding to much. Basicaly
addition of EDTA does not affect binding of 6-His tag to Ni-NTA at all :-),
but what it does affect is the binding Ni ions to NTA-agarose. So, by
adding EDTA you simply decrease amount of Ni ions boud to NTA-agarose.
Ni-NTA agorose supplied by QIAGEN contains 8-12 umol Ni2+/ml of the gel.


Nikolai
http://128.252.119.253





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