Non-radioactive PCR

rvdeshp0 at INTNET.UPJ.COM rvdeshp0 at INTNET.UPJ.COM
Thu Aug 10 15:42:55 EST 1995


I would like to qualitatively determine the positivity or negativity of a
sample for PCR with a given set of primers.  I plan to use biotinylated or
fluorescinated primers (or a combination of both) for PCR, and detect the
final product in an ELISA using streptavidin-coated plate and
anti-fluorescin-AP conjugate.  There are a few papers in the journal -
Biotechniques, that describe parallel procedures.  Does anyone have any
suggestions on the "do's and don'ts" of such techniques ?  Also, it would be
of great help to know how to avoid picking up signal from false-positive
PCR by-products ?  Any help will be greatly appreciated.  Thanks.




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