dNTP mixes and smears

Robert M Bellin rbellin at iastate.edu
Thu Aug 10 14:02:32 EST 1995


Hello all,
	
	Lately we have noticed a strange thing in our lab.  It seems
that if we freeze dNTPs as a mix, we get large smears in our PCR
reactions, even without template present.  But if the dNTPs from the same
stock vials are frozen separately, and added individually to the PCR
reactions, no smears result.  We have also noticed this with one
commercial mixture of dNTPs (which was supplied to us as a mixture).  Has
anyone else ever seen anything like this?

Thanks,

Rob Bellin
rbellin at iastate.edu  



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