Losing pieces of plasmid
Merideth Kelley
mlkelley at u.washington.edu
Thu Aug 10 12:22:44 EST 1995
Does anyone have a suggestion that will keep my E. coli from spitting out
pieces of plasmid? I'm trying to put 6kb into a 3kb vector that already
has 10kb inserted. Clones isolated after the ligation don't look like
either the parent or the predicted product. I know that 19kb is pushing
it as far as plasmid size, but I'd appreciate any advice.
Thanks
MLK.
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