pcr from lambda-gt11

Shoumo Bhattacharya sbhattac at mbcrr.harvard.edu
Thu Aug 10 14:53:46 EST 1995


Hi,

What are the best oligo pairs and reaction conditions for amplifying
the inserts from gt11 library clones?  Also has anyone used 'long-
distance' pcr for this, and is it any better? If so what oligos
and conditions did you use?

Many thanks.

Shoumo Bhattacharya
Dana-Farber Cancer Institute





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