direct PCR from bacterial colonies.

Russ Boggs rboggs at isisph.com
Thu Aug 10 17:51:50 EST 1995


In article <40852g$n1t at fs2.abdn.ac.uk>, bch127 <bch127> wrote:

> Does anyone have a method for PCr amplification of a plasmid from bacteria
> scraped off a plate into a 96-well plate as an alternative to minipreps, i.e
> how do you make sure that the bacteria lyse ? is 5 minutes at 95 C
enough or do
> you have to do anything else ? I'm sure I heard someone talking about
this once
> but I cant remember where.
>                 Neil Taylor bch127 at abdn.ac.uk

I routinely use a pipet tip touched to a colony and swirled briefly in the
PCR mix in PCR tube.  DH5-alpha cells.  5 minutes at 94°C.  It works
great.
--Russ



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