rcaspi at sdcc3.ucsd.edu
Thu Aug 10 12:08:13 EST 1995
This is a dumb question, but I might as well ask it:
I need to design oligonucleotides, that will be used mainly as primers
for cycle sequencing.
I have a program that can analyze the oligos (vectorNTI), and provide
thermodynamic data about the stability of possible dimers and hairpin
loops. My problem is - what are acceptable numbers: for example, if
the program anticipates a dimer with dG of 0.4Kcal/mol, is this
acceptabe for my purpose, or not?
And what if I use these oligos for radioactive probing, what are the
limits in that case?
Thank you for any input on this issue
Scripps Institution of Oceanography Phone (619) 534-0638
La Jolla CA 92093-0202 Fax (619) 534-7313
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