separating 2,8 kb from 3,0 kb

Andreas Enger gkd2 at mailserv.uni-giessen.de
Fri Aug 11 06:52:15 EST 1995


my problem is to separate the products of a digestion: two dna-fragments of 
2,8 kb and 3,0 kb, respectively. i tryed different concentrations of 
agarose, but the 
separation is always very poor. does anyone have a suggestion or a method?
should i try polyacrylamide - tae - gels? what concentration then, and 
which method of dna-purification?


thanks

Andreas Enger

Kerckhoff-Klinik, MPI
Hemostasis Res. Unit
Sprudelhof 11
61231 Bad Nauheim

Tel: +49-6032-996-714
Fax: +49-6032-996-707
Lab: +49-6032-996-713

Private: +49-641-73814

Seltersweg 18
35390 Giessen






More information about the Methods mailing list