separating 2,8 kb from 3,0 kb

[Andreas Enger]

my problem is to separate the products of a digestion: two dna-fragments of 
2,8 kb and 3,0 kb, respectively. i tryed different concentrations of 
agarose, but the 
separation is always very poor. does anyone have a suggestion or a method?
should i try polyacrylamide - tae - gels? what concentration then, and 
which method of dna-purification?


thanks

Andreas Enger

Kerckhoff-Klinik, MPI
Hemostasis Res. Unit
Sprudelhof 11
61231 Bad Nauheim

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