dNTP mixes and smears

Lee Smith Lee.Smith at bbsrc.ac.uk
Fri Aug 11 04:55:41 EST 1995


I use PrimeZyme from Biometra which comes with
a dNTP mix. At first, all my PCRs were fine, but
now everything is smeared: even samples which previously
amplified OK.
 Could the dNTPs be the problem - I've tried switching
batches; I've also tried storing at +4C.
I've changed every single component and still get
smears everywhere.
HELP!!!!!

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Lee Smith
IAH Compton,UK
Tel: 01635 578411 x2598
E-Mail: Lee.Smith at bbsrc.ac.uk





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