MELTING CURVES, DGGE, SOFTWARE OH MY!
affouj at rpi.edu
Thu Aug 10 11:14:31 EST 1995
hi. i've begun using DGGE separation on PCR-amplified 359bp
fragments, and am interested in any software that might be
available for calculating melting maps of oligos...does
anyone have any info about this? i know there are commercial
packages available (i.e. biorad's macmelt- $500!!!) but the
prices are a bit prohibitve...
any information about this would be greatly appreciated,
also any knowledge about DGGE resources in general,
especially about the using of GC clamps for optimization -
we are about to try this ourselves.
thanks very much in advance,
rensselaer polytechnic institute
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