pH growth optimum of bacteria

Ole Skovgaard olesk at RUC.DK
Fri Aug 11 05:14:30 EST 1995


In article <aha.2.0 at GERGA.SUN.AC.ZA> aha at GERGA.SUN.AC.ZA (DR A HARRINGTON, BIOCHEMIE, TEL X482 TYGB) writes:
>From: aha at GERGA.SUN.AC.ZA (DR A HARRINGTON, BIOCHEMIE, TEL X482 TYGB)
>Subject: pH growth optimum of bacteria
>Date: 10 Aug 1995 11:47:48 GMT

>Could anyone tell me how one prepares media for bacteria in order to obtain 
>different pH values? Does one use the phosphate buffer system and if so at 
>what concentration? Are there any pitfalls in the experimental design to 
>avoid?. Thanks.

Your Q is not very clear. What is your Q?
Yes there are pitfalls, for instance the growth of bacteria may generate 
either acid or ammonia dependent on the carbon source. 

Using phosphate buffered medium 100 to 200 mM P may be appropriate. If a 
cleaner buffer is desired MOPS is the choice.

yours
ole

******************************************
****   Ole Skovgaard                  ****
****   Dept. Life. Sc. & Chemistry    ****
****   Roskilde University, DK        ****
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