Direct PCR of Bacterial Colonies

[schoenfeld at MSSCC.MED.UTAH.EDU]

Hi -
I've seen a few posts on this subject, most of which involve resuspending
the colony in a buffer then using part of this suspension as a PCR
substrate.  My variation doesn't involve resuspending the colony,
minimizing manipulations:

Just stab the colony w/a toothpick and swirl it in your PCR mix.  Do a 3
min denaturation step at 94 before cycling.

Robert Schoenfeld
Univ. Utah