PCR from colonies

Volker Blaschke vblasch at gwdg.de
Fri Aug 11 08:26:47 EST 1995


Well, to check if our transfection into E. coli has worked, we resuspend 
a small pellett in 15 ul ddH2O, boil them for 5 minutes at 95 Celsius, 
spin them for 5 minutes at full speed and take 10 ul supernatant for 
PCR. Works really well and should be ok for microtiter plates as well, 
you don't really need that many cells.


Good luck,

 Volker

Dr. Volker Blaschke
Dept. of Biochemistry
AG Prof. Jungermann
Humboldtallee 23
D-37073 Goettingen
Germany

Email: vblasch at gwdg.de
Tel.: xx49-551-39-5959 / -5978
FAX :            -5960





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