Direct PCR of Bacterial Colonies

Steven Poet poets at ccmail.orst.edu
Fri Aug 11 18:46:48 EST 1995


Be careful, I've read that toothpicks can inhibit the reaction sometimes.

schoenfeld at MSSCC.MED.UTAH.EDU wrote:
>
> Hi -
> I've seen a few posts on this subject, most of which involve resuspending
> the colony in a buffer then using part of this suspension as a PCR
> substrate.  My variation doesn't involve resuspending the colony,
> minimizing manipulations:
> 
> Just stab the colony w/a toothpick and swirl it in your PCR mix.  Do a 3
> min denaturation step at 94 before cycling.
> 
> Robert Schoenfeld
> Univ. Utah
> 




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