Ethanol/Coumassie?

Klavs Dolmer klavs
Fri Aug 11 11:45:05 EST 1995


Hi

We have used ethanol in stead of methanol with great succes
for a long period of time. Stain: 0.25% CBB, 50% EtOH, 10% HAc
in water (Stain for 5 min. at 65 C or 15 min at RT), 1'st destain:
50% EtOH, 10% HAc in water (destain 5-10 min. at RT), 2'nd destain:
10% HAc in water (destain for 2-48 hours).
Using this procedure, no fading of bands is observed.

Klavs




More information about the Methods mailing list