direct PCR from bacterial colonies.

Hung Tri Nguyen Nguyenhd at hermes.bc.edu
Fri Aug 11 11:05:28 EST 1995


I normally pick the colonies with toothpick, suspend the cells in 50ul 
of water, vortex the tube, heat the tube at 99oC for 5 min and spin down 
the cells at 14,000rpm for 1 min.  I use 10ul of the supernatant for 
50ul PCR reaction. It works everytime.
Good luck!





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