Direct PCR of Bacterial Colonies
yangy at rockvax.rockefeller.edu
Sat Aug 12 22:04:14 EST 1995
I use sterilized pipetman tips and haven't had any problems. It works
really well if I stab a lot of the colony into the PCR mix, not so well if
I stab just a little bit of the colony into the PCR mix.
Steven Poet <poets at ccmail.orst.edu> wrote:
> Be careful, I've read that toothpicks can inhibit the reaction sometimes.
> schoenfeld at MSSCC.MED.UTAH.EDU wrote:
> > Hi -
> > I've seen a few posts on this subject, most of which involve resuspending
> > the colony in a buffer then using part of this suspension as a PCR
> > substrate. My variation doesn't involve resuspending the colony,
> > minimizing manipulations:
> > Just stab the colony w/a toothpick and swirl it in your PCR mix. Do a 3
> > min denaturation step at 94 before cycling.
> > Robert Schoenfeld
> > Univ. Utah
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