Transfection of cell suspensions

newaac amixson at UMABNET.AB.UMD.EDU
Sun Aug 13 21:49:46 EST 1995

   I have in the past transfected monolayer cells, but I now need to 
transfect several cell lines that are in suspension.  I have found that 
transferring the cells from the 6 well dishes to the centrifuge tubes for 
the several washes that are necessary a real pain.  Is there a way of 
transfecting these cells that would avoid all these transfers?

Jim Mixson
U. of Maryland at Baltimore 

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