Rapid DNA Ligation Kit ???

Daniela Sciaky reedan at globalone.net
Sun Aug 13 15:36:00 EST 1995


 Did anybody try out the 'Rapid DNA Ligation Kit' from Boehringer ? They 
> say Ligation in 5 minutes; well I tried, but it absolutely didn't work. 
> Has anybody made similar experiences ?? 

I've used this kit for only the easy type of ligations where ends are
compatible via restriction enzyme. Incubate for 15 minutes and it works
fine.

Daniela



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