multiple runs of PCR?

walter wlech at medicine.medsch.ucla.edu
Sun Aug 13 16:56:29 EST 1995


Rather than continuing with your current method, try designing a 
primer set interior to your current primers if specificity is your goal. 
You'll achieve an increase in sensitivity as well, due to the 
exclusion of competing non-specific templates that may be binding 
your first (outer) primer pair, making the overall process more 
efficient.

Walter Lech
UCLA Dept. of Medicine





More information about the Methods mailing list