multiple runs of PCR?
wlech at medicine.medsch.ucla.edu
Sun Aug 13 16:56:29 EST 1995
Rather than continuing with your current method, try designing a
primer set interior to your current primers if specificity is your goal.
You'll achieve an increase in sensitivity as well, due to the
exclusion of competing non-specific templates that may be binding
your first (outer) primer pair, making the overall process more
UCLA Dept. of Medicine
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