separating 2,8 kb from 3,0 kb

Hank Seifert h-seifert at nwu.edu
Mon Aug 14 14:01:11 EST 1995


In article 
<Pine.A32.3.91.950811134348.30598A-100000 at d1.hrz.uni-giessen.de>, 
gkd2 at mailserv.uni-giessen.de says...
>
>
>my problem is to separate the products of a digestion: two dna-fragments of 
>2,8 kb and 3,0 kb, respectively. i tryed different concentrations of 
>agarose, but the 
>separation is always very poor. does anyone have a suggestion or a method?
>should i try polyacrylamide - tae - gels? what concentration then, and 
>which method of dna-purification?

Try a 0.8% agarose-TAE gels run slowly with buffer recirculation.
>
>
>thanks
>
>Andreas Enger
>
>Kerckhoff-Klinik, MPI
>Hemostasis Res. Unit
>Sprudelhof 11
>61231 Bad Nauheim
>
>Tel: +49-6032-996-714
>Fax: +49-6032-996-707
>Lab: +49-6032-996-713
>
>Private: +49-641-73814
>
>Seltersweg 18
>35390 Giessen
>
>
>

-- 
Hank Seifert <h-seifert at nwu.edu>
Associate Professor
Department of Microbiology-Immunology
Searle 6-467, Maildrop W213
Northwestern University
303 East Chicago Ave.
Chicago, IL 60611
Tel: (312) 503-9788
Fax: (312) 503-9787 or -1339




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