separating 2,8 kb from 3,0 kb
h-seifert at nwu.edu
Mon Aug 14 14:01:11 EST 1995
<Pine.A220.127.116.110811134348.30598A-100000 at d1.hrz.uni-giessen.de>,
gkd2 at mailserv.uni-giessen.de says...
>my problem is to separate the products of a digestion: two dna-fragments of
>2,8 kb and 3,0 kb, respectively. i tryed different concentrations of
>agarose, but the
>separation is always very poor. does anyone have a suggestion or a method?
>should i try polyacrylamide - tae - gels? what concentration then, and
>which method of dna-purification?
Try a 0.8% agarose-TAE gels run slowly with buffer recirculation.
>Hemostasis Res. Unit
>61231 Bad Nauheim
Hank Seifert <h-seifert at nwu.edu>
Department of Microbiology-Immunology
Searle 6-467, Maildrop W213
303 East Chicago Ave.
Chicago, IL 60611
Tel: (312) 503-9788
Fax: (312) 503-9787 or -1339
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