RNA from fat tissue

Torsten Boerchers borcher at uni-muenster.de
Mon Aug 14 06:01:58 EST 1995


Hello,

I wondered whether anybody out there could direct me to a
protocol for isolation of RNA from fat tissue. I have some
info that it is mandatory to separate adipocytes from
other cells (e.g. by collagenase treatment) and to extract
lipids.
The aim is to have RNA for Northern or RT-PCR of an adipocyte
specific protein.
Any experience? Thank you very much for your help.

Torsten
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                          Torsten Boerchers                            /
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