Help: constant denaturing gel electrophoresis (CDGE)

Chao-Liang Wu, PhD wumolbio at
Mon Aug 14 06:17:31 EST 1995

Hi Netter,
I am trying to determine the hetroduplex mutant of a PCR product by CDGE 
with 50% denaturant and 60 degrees.  All I got was just a miserable 
smear.  Does anyone out there have any experence about CDGE ?  Any 
suggestions would be appreciated.

Chao-Liang Wu

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