DNA Footprinting ( using DNAase)

damania DAMANIA_B at a1.mscf.upenn.edu
Mon Aug 14 15:57:16 EST 1995


HI,

Has anyone out there ever down DNA footprinting with DNAase 1 and
has a reference for a good protocol that will work?

Also, when running a control lane with the DNA sequence of the
probe alone, do you need to the sequencing reactions using
Maxam-Gilbert method? or is it OK to do
it the Sanger way??
I was wondering if that made a difference or not..

I appreciate any input I can get..

thanks a bunch,
d.



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