Summary of strataclean prep protocol
dkim at nmsu.edu
Mon Aug 7 12:54:38 EST 1995
I have been following the Strataclean discussion a little late. Was the
original intent to find a solid phase method to remove proteins from
nucleic acid mixtures?
If so, then you might want to try adsorbing proteins to PVDF membrane. I
believe S&S has a microfuge spin cup in their Ultra-Free line that has a
PVDF membrane for this express purpose. The paper cited in the
instruction book describes an experiment in which RNAse A is loaded into
a solution, then passed through the PVDF. RNA added to the flow-through
is not degraded, even after extensive incubation, showing that the RNAse
is completely removed by this procedure.
(Don't ask me for the citation . . . I can't remember it. Call S&S
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