Help with PLP Mutagenesis
Steven_Burghart at BAYLOR.EDU
Steven_Burghart at BAYLOR.EDU
Tue Aug 15 11:45:46 EST 1995
We decided to try this new mutagenesis protocol found in May 95
Biotechniques. We got high product concentrations with the first 2 PCRs
(583 bp and 984 bp) but have not been able to get the final blunt end ligated
PCR product (1576 bases).
We've tried this about 5 times first using the protocol supplied in the
Biotechniques paper and then using standard phosporylation & ligation
procedures found in Maniatis before doing the 3rd PCR.
Any ideas about why this protocol isn't working for us?
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