Xcm-1

[Don Haut]

I was just wondering if anyone had any idea why Xcm-1 is not as
"re-ligate-able" as other enzymes.  The NEB catalog states that after a
five fold over digestion with Xcm-1, >20% of the DNA fragments can be
ligated and of these >95% can be recut.  Most of the other enzymes >95% of
the DNA fragments can be ligated and recut.  I have several ideas but I
was wondering if anyone knew what is really going on.

Just wondering.

Don

Don Haut 
Molecular Microbiology and Immunology
University of Missouri-Columbia
C601591 at showme.missouri.edu
314-882-3171