Home made geneclean??
The Wagner Lab
ewlab at uci.edu
Tue Aug 15 16:39:34 EST 1995
Margon Vandongen <vando005 at mc.duke.edu> wrote:
>This is a multi-part message in MIME format.
>This is related to the next question in this group, my advice
>is not to try this on your own, it is not as simple as it seems.
>Kits are standardized and work (most times) as advertised.
>with regards, Margon Vandongen
Homemade Geneclean solutions:
NaI solution: 90.8g NaI, 0.5g Na2SO3. H2O to 100ml
Glassmilk: 12.5g silica (Sigma #5631), 25ml H2O
New Wash: 50ml 100% EtOH, 2ml 5M NaCl, 2ml 0.5M Tris pH7.5, 0.4ml EDTA
pH8, 46ml H2O
I have never had a problem with homemade geneclean. I think it is as
simple as it seems.
mdpetros at uci.edu
Molecular Biology and Biochemistry
University of California, Irvine
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