RNA from T-cells
vblasch at gwdg.de
Tue Aug 15 04:29:09 EST 1995
Dear fellow researchers,
I need to make RNA from Buffy-coat (native) T-cells. However, with all
the protocolls I've used so far (e.g. GuSCN/Ultracentrifugation,
Chomczynski/Sacchi and Qiagen RNeasy kit), yield was incredibly low
(around 5 ug total RNA from 20 million cells).
Fort Northerns, I need much more than this, but cell supply is obviously
Does anyone know if this yield is in fact what one gets from native
T-cells or of another methods giving higher yields?
Qiagen states in their protocoll that their yield is around 5 ug total
RNA from 1 million T-cells, but what they don't say (and can't say on
the phone) is if that's native cells or stimulated cells or a cell line.
Thanks for your help in advance!
Dr. Volker Blaschke
Dept. of Biochemistry
AG Prof. Jungermann
Email: vblasch at gwdg.de
Tel.: xx49-551-39-5959 / -5978
FAX : -5960
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