Why use LMP agarose gels?
vando005 at mc.duke.edu
Tue Aug 15 14:26:40 EST 1995
We routinely use Gibco BRL ultra pure agarose and have good
results with ligations, it is cheaper too.
My guess is that it is your fragment purification method rather
than your agarose that is inhibiting your ligation.,..
Did you ever try to mix it in with a single enzyme cut vector
(not compatible with your insert of course) to see if it is
inhibiting? Good luck, Margon VanDongen
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