Direct cell to cell Yeast-E.coli plasmid shuttle by electroporation?
agreene at bih.harvard.edu
Wed Aug 16 07:51:21 EST 1995
There is a protocol from the Roger Brent Lab (used for rescuing plasmids
for 2 hybrid screening) that is just what you describe. I've not tried
it--- anyone? It's available online at:
Set turboGopher or Netscape to the above address.
On Wed, 16 Aug 1995, John R. Thompson wrote:
> Has anyone mixed yeast cells and E.coli cells and electroporated to effect
> a direct cell to cell transfer for shuttling plasmids? I can't recall if
> seen the concept published or just thought it should be possible.
> John Thompson
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