Help Primer Extension

[Mr. J. Membrillo-Hernandez]

Hello,
	I have tried to identified the trascriptional start site for a 
gene in E. coli, however I have had problems doing it. I have follow the 
protocol in Maniatis et al. and did not work, could anybody help me to 
have a good protocol for primer extension?, thanks in advance.

						Jorge
						University of London