Help Primer Extension

Mr. J. Membrillo-Hernandez jmembril at
Wed Aug 16 07:40:21 EST 1995

	I have tried to identified the trascriptional start site for a 
gene in E. coli, however I have had problems doing it. I have follow the 
protocol in Maniatis et al. and did not work, could anybody help me to 
have a good protocol for primer extension?, thanks in advance.

						University of London

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