Xcm-1

Duncan Clark Duncan at genesys.demon.co.uk
Wed Aug 16 05:22:41 EST 1995


In article: <c601591-1508951026240001 at 128.206.12.143>  c601591 at mizzou1.missouri.edu (Don Haut) writes:
> 
> I was just wondering if anyone had any idea why Xcm-1 is not as
> "re-ligate-able" as other enzymes.  The NEB catalog states that after a
> five fold over digestion with Xcm-1, >20% of the DNA fragments can be
> ligated and of these >95% can be recut.  Most of the other enzymes >95% of
> the DNA fragments can be ligated and recut.  I have several ideas but I
> was wondering if anyone knew what is really going on.
> 
> Just wondering.

If you look at any palindromic RE that leaves a single base overhang you 
will find that they all ligate poorly if at all. I don't know if it is
the kinetics of the ligase or what - just a fact of RE's.

Duncan

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