Need colorimetric assay to detect PCR products.
dkim at nmsu.edu
Tue Aug 8 12:05:17 EST 1995
WRT quick assay for the presence of PCR products:
I believe one can simply add ethidium bromide to the reaction mixes, and
assay for UV-induced fluorescence. The ethidium bromide does not
interfere with the PCR reaction. Fluorescence from primers and dNTPs is
very low, while fluorescence increases with the presence of dsDNA
products. It is even possible to assay the progress of the reaction in
real time, with the right equipment. If one did a 96-well array of
reactions (e.g. in a PE 9600 machine), including ethidium bromide, then
placed the array of tubes on a transilluminator, one could photograph the
array from the top, giving a nice +/- assay of DNA synthesis in each tube.
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