Bruce D. Erickson
berickson at fdant.nctr.fda.gov
Wed Aug 16 08:13:39 EST 1995
In article <3vmdgd$d9d at mark.ucdavis.edu> szeliasf at chip.ucdavis.edu (Elias Fernandez) writes:
>would anyone have carried out a mutagenesis where a single residue was
>ommited in the coding gene?
>would it be possible to use a primer with a codon missing and generate
>the mutant by pcr amplification so that the protein has a residue missing?
I haven't tried it with PCR, but it worked just fine using an
oligonucleotide-mediated mutagenesis system (in my case the Transformer system
form Clontech). I used a 20-mer oligo with ten nucleotides on either side of
the three nucleotide gap. I don't recall what the efficiency was, but I got
the clone in the first set of mutants that I screened.
(No affiliation with Clontech)
> Elias J. Fernandez |
> University of California | ## ## "It's not just an adventure;
> Section of Molecular and | (@ (@ it's a job!"
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> Tel. No. : (916)-752-3356 | ~\_====+
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> e-mail : ejfernandez at ucdavis.edu
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