Tm's of oligo dT primers
sullivan at gwis2.circ.gwu.edu
Wed Aug 16 15:51:03 EST 1995
Do the calculated Tm's of oligo dT-containing primers (15-20 T's) bear
*any* resemblance to their actual performance at different PCR annealing
temperatures, in anyone's experience?
And (I know someone asked this recently, but I've lost the thread) when
factoring salt concentration into a Tm calculation, does one include all
cationic species (K+, Mg++, Tris?) in the calculation or just Mg++?
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