Direct cell to cell Yeast-E.coli plasmid shuttle by electroporation?

AL R. PLUMMER aplummer at sprint.uccs.edu
Wed Aug 16 10:37:55 EST 1995


John R. Thompson (jrt at intac.com) wrote:
: Has anyone mixed yeast cells and E.coli cells and electroporated to effect
: a direct cell to cell transfer for shuttling plasmids?   I can't recall if
: seen the concept published or just thought it should be possible.

: John Thompson

Look at the article:

Marcil,R. and Higgins,D.R. (1992)  Direct transfer of plasmid DNA from
yeast to E.coli by electoporation.  Nucleic Acids Res. 20:917

----------------Al (aplummer at sprint.uccs.edu)



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