EtBr staining of RNA

D L Clarke mb935645 at silver.shef.ac.uk
Thu Aug 17 11:05:35 EST 1995


I have ran a 2% w/v  formamide gel for a Northern blot.
However, when I stained the markers in 1ug/ml EtBr I could see
nothing after a 5min incubation. I know I could have degraded
my RNA but I would expect to see some sort of smear even
if it has degraded.  Is single stranded impossible to visualise
with EtBr?  Should I incubate for longer or change from
DEPC water to TAE or TBE during the incubation.

Any suggestions would be gratefully accepted

Thanks

Dave Clarke (D.L.Clarke at sheffield.ac.uk) 




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