PCR from colonies

M.J. Lush mjl11 at le.ac.uk
Fri Aug 18 08:39:06 EST 1995


	I transfer a colony into 30 ul of dH2O put 15ul into 5ml of 
LB broth (for miniprep and glycerol stock) and heat the rest at 
95C for 5 minutes and use it directly in my PCR.

(needless to say it works just fine :-)

-- 
Michael 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
NPC rights activist  | Nameless Abominations are people too! 



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