EtBr staining of RNA

Nigel Kenward mbxnjk at vax.nott.ac.uk
Fri Aug 18 02:54:19 EST 1995


Hi,
We put EtBr (1 microlitre of 10mg/ml is LOADS) in our samples before 
loading.
Other things we tried include the method you use, but it took a 20 
minute incubation and about 10  min destain to see bands / smears well.

Good luck,
Nigel Kenward

Nigel Kenward              TEL:+44 (0)115 9249924 ex. 44789
Dept. of Biochemistry      FAX:+44 (0)115 9422225
Queens Medical Centre      
Clifton Boulevard          
Nottingham						
U.K.                   "Back off man, I'm a scientist!"  -- Bill Murray.





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