Linkers from cDNAs?

the End jgraham at bronze.ucs.indiana.edu
Fri Aug 18 09:38:55 EST 1995


Hello again,

Has anyone found a reliable way of seperating unligated linkers
from cDNA prior to vector ligations? I have a non-renewable 
"trace" of cDNA in a very small volume which I cannot even 
risk a precipitation, much less a gel purification step.

Sounds hopeless I know. I'm thinking spin column or Micron 
filtration unit?

Thanks much, e-mail copies please,

Jim
J. Graham
Washington University of St. Louis 



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