Dots in sequencinggels

yolanda yangy at rockvax.rockefeller.edu
Fri Aug 18 12:04:30 EST 1995


In article <Marie.Svenson-1808951359480001 at mac19.fysbot.uu.se>,
Marie.Svenson at fysbot.uu.se (Dept of Physiological Botany) wrote:

> We have a problem with wedge-sequencinggel (we use biorad system). 
> Sometimes dots are visible on the film, corresponding to upper part of the
> gel.  It seem to occur at random, not depending on any different
> runningconditions as far as we can tell. The temperature is for example
> OK.Has anyone seen the same phenomena? 
> // Marie Svenson
> (Marie.Svenson at fysbot.uu.se)
> Dept. of physiological botany, University of Uppsala

This sometimes happens when you get small air bubbles in your gel.  Try
being extra-careful when you pour your gel.

yolanda
yangy at rockvax.rockefeller.edu



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