Xcm-1

Duncan Clark Duncan at genesys.demon.co.uk
Fri Aug 18 05:36:35 EST 1995


In article: <tedm-1708951732270001 at flynn-lab.uoregon.edu>  tedm at darkwing.uoregon.edu (Ted M.) writes:
> 
> In article <c601591-1508951026240001 at 128.206.12.143>,
> c601591 at mizzou1.missouri.edu (Don Haut) wrote:
> 
> > I was just wondering if anyone had any idea why Xcm-1 is not as
> > "re-ligate-able" as other enzymes. 
> 
> 
> 
> 
> Of course Mnl I has a single base 3' overhang and its listed at 80%
> re-ligatable so maybe it doesn't hold on to the end as tightly as Xcm
> I...go figure. Now if I can just find a suitable TA type vector to cut
> with it...

The original TA kit used Hph cleaved vector leaving the correct ends. For 
those of you with some left or even with an insert one could regenerate 
the original vector etc

Duncan

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