EtBr staining of RNA
Yun-Long Du, Ph.D.
yd128 at vax.albany
Thu Aug 17 11:53:04 EST 1995
In article <40vpcf$5rn at hippo.shef.ac.uk>, mb935645 at silver.shef.ac.uk (D L
> I have ran a 2% w/v formamide gel for a Northern blot.
> However, when I stained the markers in 1ug/ml EtBr I could see
> nothing after a 5min incubation. I know I could have degraded
> my RNA but I would expect to see some sort of smear even
> if it has degraded. Is single stranded impossible to visualise
> with EtBr? Should I incubate for longer or change from
> DEPC water to TAE or TBE during the incubation.
> Any suggestions would be gratefully accepted
> Dave Clarke (D.L.Clarke at sheffield.ac.uk)
First, you did not stain long enough.
RNA does stain poorly with EtBr and you may use higher concentration of EtBr.
Even you use higher concentration and stain for longer time, you will not
expect to see strong bands like the DNA stained with EtBr. Goos Luck.
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